Figure 2.

BLI proof of concept in the healthy brain in vivo. LV-EF1α-IR particles were injected into the striatum to assess the timing for stable Luc2 expression. (A) Representative images of BLI signal behavior over time in one individual. Shown is the frame with maximal signal, overlaid with the corresponding photographs. (B) Quantitative BLI analysis of maximum total flux in photons per second (p/s). Signal reached steady state after about 14 days and remained stable until at least 35 dpi. The red line depicts the average values of all animals, and error bars indicate STDs. (C) Flow cytometry analysis of cells isolated at 2 weeks post LV injection. Numbers of both, microglia and MDMs remained independent of treatment. Irrespective of injected solutions [HBSS (ID 94) vs. LV] or insertion of a capillary [injection vs. no injection (con)], microglia numbers were similar and MDMs were less present. con, contralateral; dpi, days post injection; ipsi, ipsilateral; MDMs, monocyte-derived macrophages; STD, standard deviation.