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IHC and RNA-ISH of LV-Ym1-IR injected brains at 21 days after MCAO. IHC on top, RNA-ISH below. Iba-1 and GFAP antigens were strongly expressed in the ischemic area (Left). Some eGFP+ cells co-expressed Iba-1 (Center), but not GFAP (Right). RNA-ISH confirmed reporter gene expression. Some Luc2 RNA positive cells co-expressed Iba-1 RNA. 4X magnifications left; 60X remaining images.
