Table 3.
RNAScope probe list.
| Supplier | Conc. | |
|---|---|---|
| Probe diluent | Advanced Cell Diagnostics (ACD), Newark, CA, USA | |
| C2 (Luc2) | ACD | 1:50 |
| C3 (Iba-1) | ACD | 1:50 |
| 3-plex pos. ctrl. containing: | ACD | 1 drop |
| POLR2A (C1, low) | ||
| PPIB (C2, middle) | ||
| UBC (C3,high) | ||
| TSA plus fluorophores | ||
| TSA buffer in C1 | PerkinElmer, Waltham, MA, USA | |
| Cyanine Cy3 in C2 | PerkinElmer | 1:1,500 |
| Cyanine Cy5 in C3 | PerkinElmer | 1:750 |
| SSC buffer (20X) | ||
| NaCl | 175.3 g | |
| Na3C6H5O7 | 88.2 g | |
| dH2O | ad 800 mL | |
| Adjust pH to 7.0 with HCl, 1 M | ||
| dH2O | ad 1,000 mL |
C, channel; ctrl, control.