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. 2019 Jun 4;10:1268. doi: 10.3389/fimmu.2019.01268

Figure 1.

Figure 1

Skin samples from psoriasis patients express lower levels of TSP-1, and CD47 compared with healthy controls. mRNA levels of TSP-1 (A) and its receptor CD47 (B) were analyzed by RT-PCR in skin samples from 26 psoriasis patients and 20 healthy controls. GAPDH expression was used to normalize gene expression. Data were analyzed by one-way ANOVA followed by Tukey's multiple comparisons test, ***p < 0.001, *p < 0.05. (C) Double immunofluorescence staining of CD47 (green) and CD45 (red) in a representative skin sample from control subjects (left panels) and lesional skin from psoriasis patients (right panels) is shown. Nuclei were counterstained with DAPI (blue). (D) For quantification of immunofluorescence staining, fluorescence intensity of CD47 in CD45+ dermal cells was calculated using Image J software. (E) Representative expression of CD47 (green) in skin samples from control subjects and psoriasis patients. Graphs represent mean ± SD. Differences between groups were determined by one-way ANOVA followed by Tukey's multiple comparisons test, ****p < 0.0001, **p ≤ 0.001.