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. 2019 Jun 4;10:1253. doi: 10.3389/fimmu.2019.01253

Figure 5.

Figure 5

STAT1 modulates increased p65 recruitment to GAS/NFκB or ISRE/NFκB composite sites. (A) ChIP-PCR of STAT1 at Serpina3i, Steap4, Irf1, Ccl5, Ifit1, Gbp6, Cxcl10, and Gbp7 gene promoters (primers are listed in Table S2) in VSMC WT treated with IFNα (8 h), IFNγ (8 h), LPS (4 h), IFNα (8 h) + LPS (4 h), IFNγ (8 h) + LPS (4 h). Mean ± s.e.m., n = 2. (B) ChIP-PCR of p65 at Serpina3i, Steap4, Irf1, Ccl5, Ifit1, Gbp6, Cxcl10, and Gbp7 gene promoters in VSMC WT treated with IFNα (8 h), IFNγ (8 h), LPS (4 h), IFNα (8 h) + LPS (4 h), IFNγ (8 h) + LPS (4 h). Mean ± s.e.m., n = 2. (C) ChIP-PCR of p65 at Serpina3i, Steap4, Irf1, Ccl5, Ifit1, Gbp6, Cxcl10, and Gbp7 gene promoters in VSMC STAT1 KO treated with IFNα (8 h), IFNγ (8 h), LPS (4 h), IFNα (8 h) + LPS (4 h), IFNγ (8 h) + LPS (4 h). Mean ± s.e.m., n = 2.