Fig. 3.

Genome wide impact of palbociclib on DIPG.

(a) Heatmaps of palbociclib affected genes (Right panel) and Gene Ontology analysis for genes in the heatmap (Left panel). Heatmaps show that 306 of the genes are down-regulated, whereas 65 genes are upregulated (log2 fold change >1 or < −1, FDR < 0.05, FPKM >1 in control). Gene Ontology (GO) analysis demonstrates the pathways enriched in the differentially expressed genes (n = 2).

(b) Gene Set Enrichment Analysis (GSEA) of palbociclib-repressed genes (FDR < 0.01, p < 0.001). Heatmap shows the RNA-seq enrichment (FPKM) for genes repressed by palbociclib treatment. Color bar represents signal density distribution (n = 2).