Adhesion of P. aeruginosa, S. aureus, and 1: 1 mixed species in NB, BHI broth, LB, and RPMI 1640 media. MTT assay was performed to quantify the adherent cell mass. Adhesion of bacterial cells in PBS served as the control. The error bars represent ±2 SD. For comparison of relative bacterial adhesion in different culture media, a two-way ANOVA was performed followed by a Bonferroni post hoc test. * p < 0.05 using ANOVA, indicating a significant difference in adhesion between the four culture media for the given organism.