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. 2019 May 28;10:1142. doi: 10.3389/fimmu.2019.01142

Figure 5.

Figure 5

Lung DCs from gut microbiota ablated mice fail to stimulate Mtb specific naïve CD4 T cells. Mice treated with Abx were infected with Mtb and orally administered TDB as illustrated in the legend to Figure 1. After 3 wk, sorted CD11c+ lung cells enriched for DCs were pulsed with PPD (15 μg/ml) overnight prior to coculture with Mtb specific naïve CD4 T cells which were prior labeled with CFSE (2 μM) at a ratio of 1:10. After 72 h, proliferation of CD4 T cells was examined by flow cytometry. (A) Contour plots and (B) Bar graph represent the percent population of CFSE+ CD4 T cells. Later, (C) IFN-γ, (D) IL-17, and (E) IL-10 level was measured in the culture SNs by ELISA. Data are shown as mean ± SD of 2–3 independent experiments (n = 6 mice/group). **p < 0.01, ***p < 0.001.