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. 2019 May 21;10:608. doi: 10.3389/fphys.2019.00608

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Copyright © 2019 Chen, Fu, Song and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HULC, UCA1, and MALAT-1 lncRNAs were upregulated following LPS induced sepsis in HMECs. Nuclear extracts prepared from untreated or LPS treated HMECs were subjected to immunoprecipitation using anti-hnNRP K and anti-coREST (A) antibodies. RNA was isolated from immunoprecipitates and subjected to quantitative RT-PCR to detect relative enrichment of indicated lncRNAs in anti-hnRNP K immunoprecipitate (B) and anti-coREST immunoprecipitate (C), compared to IgG immunoprecipitates. Data represent relative enrichment compared to IgG precipitates. ^∗P < 0.05 compared to untreated cells.