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. 2019 May 31;10:620. doi: 10.3389/fphar.2019.00620

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Copyright © 2019 Zhang, Han, Xue, Deng, Wu, Peng, Zhang, Xuan, Pan and Fu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MLB inhibited inflammation via Jak2/Stat3 in RAW264.7. (A) Cell viability was examined using CCK-8. (B) The NO production was measured by Griess assays in cell media, and the levels of IL-6 (C) and TNF-α (D) were ascertained by ELISA. (E–G) The levels of the Jak2/Stat3 signaling pathway were measured by Western blotting. All samples were from 5 independent experiments. Each value is expressed as mean ± SD; n = 5 for each group; ns: no statistical significance; ^#P < 0.05, and ^###P < 0.001 versus NC group; *P < 0.05, **P < 0.01, and ***P < 0.001 versus LPS group.