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. 2019 May 23;10(6):1479–1488. doi: 10.1111/1759-7714.13097

Figure 2.

Figure 2

Expression and localization of CBLL1 in HBE and lung cancer cell lines A549 and H1299. CBLL1 expression levels were significantly altered after CBLL1 transient transfection and siRNA interference in A549 and H1299 cells. (a) Western blot analysis showed the protein expression of CBLL1 in HBE and NSCLC. (b) Immunofluorescence results showed that CBLL1 was expressed in both cytoplasm and nucleus in A549 and H1299 cells. (c) Realtime RT‐PCR results showed that CBLL1 mRNA significantly decreased after transfection with CBLL1 siRNA in A549 and H1299 cells (P < 0.01). (d) Western blot results showed that CBLL1 protein was decreased after the transfection of CBLL1 siRNA (P < 0.01).