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. 2019 Jun 4;10:660. doi: 10.3389/fpls.2019.00660

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Copyright © 2019 Aung, Masuda, Nozoye, Kobayashi, Jeon, An and Nishizawa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Plant appearance and growth of NT and OsNAS3 knockout rice. (A,B) Appearance of OsNAS3 knockout plants and non-transformants (NT) under control (A) or Fe excess (B) conditions after 39-day treatment. (C,D) Shoot length under control (C) or Fe excess (D) conditions. (E,F) Root length under control (E) or Fe excess (F) conditions. (G,H) Dry weights of shoots (G) and roots (H) under control and Fe excess condition at 23 days after transplanting (from 2nd experiment). Error bars represent the standard error (SE) of biological replicates, n = 4 for NT and n = 3 for knockout plants. Plants were grown hydroponically under control (×1 Fe) or excess ferrous Fe (×70 Fe) conditions at pH 4.0. Asterisks indicate significant differences compared to the NT at each time point or each Fe condition (^∗P < 0.05, ^∗∗P < 0.01).