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. 2019 May 22;10:1137. doi: 10.3389/fimmu.2019.01137

Figure 4.

Figure 4

Partially complement-dependent release of pro-inflammatory cytokines in C-SVMP-treated human whole blood. Blood samples were collected in the presence of lepirudin and pre-incubated with diluent buffer, compstatin or control peptide for 30 min. Then, PBS, LPS, or C-SVMP was added, and the samples were further incubated for 30 min. Plasma levels of IL-1β (A), IL-6 (B), and TNF-α (C) were determined using the BDTM Cytometric Bead Array (CBA) Human Inflammatory Cytokines Kit according to the manufacturer's instructions. Data represent the mean ± S. D. of four independent experiments. *p < 0.05 compared to the respective PBS treatment. &p < 0.05 between the LPS and C-SVMP treatments. #p < 0.05 between the compstatin and diluent buffer pretreatments.