Skip to main content
. 2019 May 28;2019:6953963. doi: 10.1155/2019/6953963

Table 1.

Primer sequences for RT-PCR.

Genes Primer sequence Annealing temp. (°C) Amplicon size (bp)
TNF-α FOR: AACTCCAGGCGGTGCCTATG 63 242
REV: TCCAGCTGCTCCTCCACTTG
IL-1β FOR: AGCTTCAGGCAGGCAGTATC 61 215
REV: TCATCTCGGAGCCTGTAGTG
IL-6 FOR: AAGTCCGGAGAGGAGACTTC 58 487
REV: TGGATGGTCTTGGTCCTTAG
15-lox-1 FOR: GGAGGAGGAACTGGAAGAA 66 704
REV: TCAGAAGATGAGCCTGTAGC
iNOS FOR: AGGAGGAGAGAGATCCGATTTAG 62 405
REV: TCAGACTTCCCTGTCTCAGTAG
Arg-1 FOR: GAAGAACCCACGGTCTGTGG 60 118
REV: TCCAACTGCCAGACTGTGGTC
U6 FOR: CTCGCTTCGGCAGCACA 60 94
REV: AACGCTTCACGAATTTGCGT