Skip to main content
. 2019 Apr 25;123(22):4705–4717. doi: 10.1021/acs.jpcb.9b01236

Figure 2.

Figure 2

Two-photon action cross section spectra and absorption polarization ratios of (a) NADH and (b) NADPH in aqueous HEPES buffer. No differences in the 2PA absorption characteristics of NADH and NADPH were evident, including in their relative susceptibility to circularly and linearly polarized excitation which remained invariant to excitation wavelength across the two-photon resonance, averaging 0.776(±0.002). The two-photon action cross sections peak at 690 nm at a value of 0.15 GM. This occurs at a similar energy to the 1PA spectrum50,58 (c and d).