Table 1. Biexponential Fitting Parameters of Fluorescence Intensity Decays of NADH and NADPH in Aqueous Buffer, across the Tuning Spectrum of the Ti:Sapphire Laser and with Single-Photon Excitation at 360 nm for Comparison.
linear | circular | ||||||
---|---|---|---|---|---|---|---|
λ/nm | α1 | τ1/ns | τ2/ns | α1 | τ1/ns | τ2/ns | |
NADH | 705 | 0.73(±0.06) | 0.344(±0.009) | 0.69(±0.02) | 0.69(±0.05) | 0.315(±0.007) | 0.70(±0.01) |
715 | 0.86(±0.06) | 0.367(±0.009) | 0.93(±0.08) | 0.78(±0.09) | 0.34(±0.02) | 0.79(±0.06) | |
725 | 0.86(±0.02) | 0.380(±0.004) | 0.78(±0.01) | 0.72(±0.03) | 0.330(±0.005) | 0.68(±0.01) | |
735 | 0.83(±0.02) | 0.366(±0.004) | 0.77(±0.01) | 0.78(±0.03) | 0.350(±0.005) | 0.76(±0.01) | |
745 | 0.82(±0.02) | 0.378(±0.004) | 0.78(±0.01) | 0.86(±0.03) | 0.391(±0.004) | 0.84(±0.02) | |
755 | 0.87(±0.03) | 0.395(±0.005) | 0.84(±0.03) | 0.80(±0.03) | 0.357(±0.006) | 0.78(±0.02) | |
765 | 0.88(±0.02) | 0.396(±0.004) | 0.90(±0.03) | 0.82(±0.03) | 0.365(±0.005) | 0.81(±0.02) | |
mean | 0.85(±0.01) | 0.379(±0.002) | 0.78(±0.01) | 0.79(±0.01) | 0.357(±0.002) | 0.74(±0.01) | |
360 | 0.83(±0.02) | 0.382(±0.005) | 0.72(±0.02) | – | – | – | |
NADPH | 705 | 0.8(±0.2) | 0.41(±0.03) | 0.9(±0.1) | 0.7(±0.1) | 0.27(±0.01) | 0.73(±0.01) |
715 | 0.79(±0.05) | 0.349(±0.008) | 0.72(±0.02) | 0.76(±0.06) | 0.321(±0.009) | 0.72(±0.02) | |
725 | 0.80(±0.06) | 0.36(±0.01) | 0.73(±0.03) | 0.7(±0.1) | 0.29(±0.01) | 0.68(±0.02) | |
735 | 0.81(±0.04) | 0.354(±0.007) | 0.78(±0.02) | 0.68(±0.08) | 0.285(±0.009) | 0.72(±0.02) | |
745 | 0.83(±0.04) | 0.373(±0.007) | 0.77(±0.02) | 0.79(±0.05) | 0.327(±0.009) | 0.73(±0.02) | |
755 | 0.86(±0.05) | 0.40(±0.01) | 0.84(±0.05) | 0.80(±0.04) | 0.348(±0.008) | 0.79(±0.02) | |
765 | 0.81(±0.06) | 0.37(±0.01) | 0.83(±0.03) | 0.75(±0.06) | 0.319(±0.009) | 0.78(±0.02) | |
mean | 0.82(±0.02) | 0.366(±0.003) | 0.77(±0.01) | 0.76(±0.02) | 0.315(±0.003) | 0.73(±0.01) | |
360 | 0.85(±0.03) | 0.378(±0.006) | 0.77(±0.02) | – | – | – | |
combined | 2PA | 0.84(±0.01) | 0.374(±0.002) | 0.78(±0.01) | 0.78(±0.01) | 0.346(±0.002) | 0.73(±0.01) |
1PA | 0.84(±0.02) | 0.380(±0.004) | 0.74(±0.01) | – | – | – |