Table 3. Tensor Components for the Two Fluorescence Decay Components of NADH and NADPHa.
| short
lifetime species |
long lifetime
species |
homogeneous treatment |
||||
|---|---|---|---|---|---|---|
| S | D | S | D | S | D | |
| NADH | –0.06(±0.02) | –0.02(±0.05) | –0.33(±0.05) | 0(±0.1) | –0.09(±0.01) | –0.03(±0.05) |
| NADPH | –0.07(±0.05) | 0(±0.1) | –0.29(±0.07) | 0.1(±0.2) | –0.07(±0.03) | 0(±0.1) |
| combined | –0.06(±0.02) | 0(±0.04) | –0.32(±0.04) | 0.1(±0.1) | –0.09(±0.01) | –0.03(±0.05) |
a
In both molecules, the long lifetime species exhibits a significant negative diagonal element, in contrast to the primarily single element short lifetime species. These differences could not be observed if the analysis assumed a homogeneous system. As the tensor components of the two molecules are identical, suggesting that the phosphate group which differentiates between them plays no role in the transition, the datasets were combined to reduce uncertainties before plotting in Figure 6.