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. 2019 May 28;2019:4348973. doi: 10.1155/2019/4348973

Figure 4.

Figure 4

Effect of PI3K inhibitor on carnosine effect against HG-induced apoptosis. (a) Apoptosis of MPC5 cells was detected by TUNEL assay (n=3). (b-d) The protein expression levels of AKT, P-AKT, Nrf2, HO-1, Bax, Bcl-2, and Cleaved caspase3 were detected by Western blot (n=3). (f) The mRNA expression of Nrf2 and HO-1 analyzed by RT-qPCR (n=3). NG: normal glucose 5.5mM; LY294002: 20μM; HG: high glucose 30mM; HG+CA: high glucose (30mM) plus carnosine (20mM); HG+CA+LY: high glucose (30mM) plus carnosine 20mM) plus LY294002 (20μM). Data are presented as mean ± SD. ∗P < 0.05, ∗∗P < 0.01 vs. NG; #P < 0.05, ##P < 0.01 vs. HG+CA.