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. 2019 Jun 11;18:192. doi: 10.1186/s12936-019-2817-8

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The current method outperforms Qiagen based kits for amplification of k13 from dried blood spots. a The optimized protocol (compatible with Omega EZ and Nunc 96-well DNA plates) is able to amplify k13 from all 5000 parasites/mL DBS samples, whereas Qiagen QIAamp and Investigator kits cannot. b Nunc DNA plates show improved sensitivity for amplification of 1000 parasites/mL DBS samples than Omega EZ DNA plates. M marker, GuSCN guanidine thiocyanate, ISOH isopropanol