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Addition of 16.7% isopropanol to the lysis buffer improves recovery of parasite DNA
| 3M GuSCN | |||
|---|---|---|---|
| ISOH | 10% | 16.7% | 25% |
| Average Ct for Pf 18S rDNA (± SD) | 33.9 (± .4) | 27.7 (± .2) | 29.4 (± .2) |
| Average Ct for human actin (± SD) | 27.0 (± .4) | 26.5 (± .2) | 26.8 (± .4) |
Experiments done with samples at parasite density of 10,000 parasites/mL
Pf, Plasmodium falciparum; GuSCN, guanidine thiocyanate; ISOH, isopropanol; SD, standard deviation; Ct, cycle threshold
