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A pH 6.5 or below for the lysis buffer leads to improved recovery of parasite DNA
| pH | 7.5 | 6.5 | 5.5 |
|---|---|---|---|
| Average Ct for Pf 18S rDNA (± SD) | 28.6 (± .6) | 27.6 (± .1) | 27.7 (± .3) |
| Average Ct for human actin (± SD) | 26.2 (± .2) | 26.2 (± .2) | 26.6 (± .6) |
Experiments done with samples at parasite density of 10,000 parasites/mL
Pf, Plasmodium falciparum; GuSCN, guanidine thiocyanate; ISOH, isopropanol; SD, standard deviation; Ct, cycle threshold
