Table 4.
Incubation at 65 °C for 1 h is superior to overnight incubation for recovery of parasite DNA from dried blood spots
| Incubation time at 65 °C | 1 h | Overnight | ||
|---|---|---|---|---|
| Lysis buffer | 3 M GuSCN | 3 M GuSCN + 16.7% ISOH | 3 M GuSCN | 3 M GuSCN + 16.7% ISOH |
| Average Ct for Pf 18S rDNA (± SD) | Undetectable | 30.0 (± .3) | Undetectable | Undetectable |
| Average Ct for human actin (± SD) | 29.8 (± .4) | 28.3 (± .3) | 28.4 (± .3) | 26.5 (± .9) |
Experiments done with samples at parasite density of 2000 parasites/mL
Pf, Plasmodium falciparum; GuSCN, guanidine thiocyanate; ISOH, isopropanol; SD, standard deviation; Ct, cycle threshold