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The current protocol outperforms Qiagen QIAamp for purification of parasite DNA from dried blood spots
| Method | Current protocol | Qiagen QIAamp | ||
|---|---|---|---|---|
| Filter paper | Whatman 3MM | Whatman 903 PS |
Whatman 3MM | Whatman 903 PS |
| Average Ct for Pf 18S rDNA (± SD) | 30.2 (± .1) | 30.2 (± .2) | 35.1 (± .1) | 35.0 (± .1) |
Experiments done with samples at parasite density of 2000 parasites/mL
Pf, Plasmodium falciparum; PS, protein saver; SD, standard deviation; Ct, cycle threshold
