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. 2019 Jun 11;20:475. doi: 10.1186/s12864-019-5869-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Validation of microarray results by qPCR analysis. a Data are expressed as fold change with respect to the control group (experimental condition appearing as subscripts). Statistical significance (N = 6–15; Student’s t-test, P < 0.05 at the α level of 0.05) is highlighted with red (up-) or green (down-regulated) background. RR, restricted ration; NR, normal ration. b Correlation of microarray and qPCR. Comparison of changes in gene expression (represented as log2) derived from qPCR and microarray hybridizations revealed a significant correlation (Spearman’s rank test, P = 0.003 at the α level of 0.05)