Table 2.
Primers used for gene expression studies by qPCR analysis
| Gene description | Target gene | Primers | Primer sequence (5′ → 3′) |
|---|---|---|---|
| Anti-Müllerian hormone | amh | Fw | CAGTCACTCTCTGCAGCCTTACAA |
| Rv | CAACATTGAATCTCCATTTCAGTTTAC | ||
| Probe | TTTGCCCTCGGGTTGCTTTCCTGTT | ||
| Calreticulin precursor | calr | Fw | ACGCCGCTGACTCTACCATCT |
| Rv | TCACATCATCTGAGACCAGGAAGTT | ||
| Catenin beta-1 | ctnnb1 | Fw | CCCCAGGCGACAGCAAT |
| Rv | CCAAATCACAATGCAGGTTGA | ||
| Cryptochrome-1 | cry1 | Fw | AGGTCAGCCGGCCAACA |
| Rv | AACGGCTCAGAGTCACACTCAAA | ||
| Endoplasmin precursor | hsp90b1 | Fw | TGAAGCGGATTTGGTTTATTGG |
| Rv | CGCCGTCAATGTCAAGTTCAT | ||
| Eukaryotic 18S ribosomal RNA | 18S | TaqMana | proprietary information |
| Follicle stimulating hormone subunit beta | fshb | Fw | TCACGGAGGCATCACCATCA |
| Rv | GCTCTTGGCAACGGGTATGA | ||
| Probe | ACCTGCGCCGGCCTGTGC | ||
| Frizzled-8 precursor | fzd8l | Fw | GGAGCGACCCATTATTTTCCT |
| Rv | CCGGCAATTAGTCTGACAATGTAA | ||
| G1/S-specific cyclin-D1 | ccnd1 | Fw | AAGCAAATCCTGTGCAAGCA |
| Rv | CGGAGGGTTGGCAATGAA | ||
| Gamma-crystallin M2 | crygm2 | Fw | CCACCTTCTACGAGGACAGGAA |
| Rv | TGGCACCTGCTCATGTAGGA | ||
| Gap junction alpha-1 protein | gja1 | Fw | CCTAAGGGCTCTCTTCTTACTTCTGA |
| Rv | CACTCCAGTCACCCATGATGAA | ||
| Geminin | gmnn | Fw | TGCAAGTCCTCCAGCAAGCT |
| Rv | CCCTTGTTCTGCATTCCACTGT | ||
| Gonadotropin releasing hormone receptor 4 | gnrhr4 | Fw | TCAACCCACTGGCGATCAAT |
| Rv | CGTGATGGTCACACTGTGGAATA | ||
| Probe | AGTGTGATTCTGTCTGTTCCCCAGATGCTG | ||
| Gonadotropin subunit beta-2 | lhb | Fw | ACACTGCCCATCAGGACACAA |
| Rv | GGGAGATCAAGGTGCCTACATG | ||
| Growth-regulated alpha protein-like | LOC106577834 | Fw | TCATCATGAATACTGCAATGACTGTT |
| Rv | TATCCTCTCCCTTGTATCATGCAGAT | ||
| Insulin-like 3 (Leydig cell) | insl3 | Fw | CTCCGGAGCTTGGACAACAC |
| Rv | AGTCCTCAGGTTGGCAAATTGAT | ||
| Insulin-like growth factor 3 | igf3 | Fw | GACCGACCGACAAGATGCA |
| Rv | TGCAAGGCACAATATGGAGTACA | ||
| Noggin | nog | Fw | GATGGAGGTGCCTGCAGAGA |
| Rv | TTCAGTTCGAGCAGGAGCATTT | ||
| Olfactomedin-like protein 2B precursor | olfml2b | Fw | CGAAGGGCAACGGAAATGTA |
| Rv | ATCCAGTTGTACGGCAGCTTGT | ||
| Prostaglandin E synthase 3 | ptges3 | Fw | CCGTGGCTGAGGCTAACAAA |
| Rv | CCTCCCAGTCTTTCCAGTTGTTAA | ||
| Testin | tes | Fw | CGTGTCTGAAATGCAAGGACAA |
| Rv | GTGGTCCGTAAGCCCACACTT | ||
| Vasotocin-neurophysin VT 2 precursor | neu4 | Fw | GGACACTGCGCTGCAACA |
| Rv | GTACCGACTCTCCCCCAAACA | ||
| Wnt-11 precursor | wnt11l | Fw | CCCCAGGCCAGCACACTA |
| Rv | ACCACCTGCAGAGCCTCACT |
a20X eukaryotic 18S rRNA pre-developed TaqMan assay (4352930E; Applied Biosystems). Fw Forward, Rv Reverse