Table 2.
Pyrosequencing analysis of individual CpGs in whole cord blood, CD14+ monocytes, and CD235+ nRBCs
| Chromosome position | Whole cord blood | CD14+ monocytes | CD235a+ nRBCs | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| chr5:373378 | chr5:373398 | chr5:373476 | chr5:373490 | chr5:373378 | chr5:373398 | chr5:373476 | chr5:373490 | chr5:373378 | chr5:373398 | chr5:373476 | chr5:373490 | |
| Methylation (%), nonsmoker (n = 19), mean ± SEM | 77.5 ± 1.3 | 78.6 ± 1.4 | 78.0 ± 1.8 | 67.5 ± 1.8 | 77.5 ± 1.6 | 79.5 ± 1.7 | 78.9 ± 2.0 | 65.6 ± 2.0 | 60.3 ± 1.9 | 61.1 ± 1.9 | 60.4 ± 2.1 | 52.5 ± 2.0 |
| Methylation (%), smoker (n = 34), mean ± SEM | 68.1 ± 1.0 | 69.4 ± 1.0 | 65.9 ± 1.4 | 54.6 ± 1.4 | 65.7 ± 1.2 | 68.2 ± 1.3 | 63.9 ± 1.5 | 49.0 ± 1.5 | 52.6 ± 1.4 | 53.3 ± 1.4 | 50.4 ± 1.6 | 39.6 ± 1.5 |
| Delta methylation (%) | − 9.4 | − 9.2 | − 12.2 | − 12.9 | − 11.8 | − 11.3 | − 15.0 | − 16.7 | − 7.7 | − 7.8 | − 10.0 | − 12.9 |
| p value (two-sided t test) | 7.0E−09* | 7.8E−08 | 5.5E−08 | 1.8E−07 | 5.7E−09 | 7.8E−08 | 2.2E−09 | 7.6E−10 | 4.8E−04 | 4.8E,04 | 8.5E−05 | 3.2E−07 |
*The statistical significance cutoff following adjustment for multiple comparisons is p < 0.0125