Fig. 1.

Validation of TSS-RNA sequencing in rat neural precursors. a. A scheme of an updated protocol for the preparation of Start-seq libraries for Illumina sequencing. b. UCSC browser shot of highly expressed Actb gene showing tracks from this study including 5′-tracks for TSS-RNA for each strand (red and blue) and mRNA-sequencing (black), alongside Pol II ChIP-seq (using an antibody against the Pol II N-terminus) track from mature rat neurons [10] representing a rat Pol II dataset that is most closely related to the current cell type. c. A zoomed-in view of Actb promoter-proximal region showing 5′- (red) and 3′-end (gold) of TSS-RNAs on this gene. The annotated Actb TSS is shown in blue bar and is located 2 bp downstream of the 5′ TSS-RNA peak. d. Correlation plot for promoter-proximal counts between two independent biological Start-Seq replicates. TSS-RNAs on the gene (sense) strand were counted in a +/− 500 bp interval from each RefSeq-annotated TSS