Fig. 2.

PLEK2 promoted the migration, invasion and metastasis of GBC cells. a, c Migration and invasion ability of NOZ and GBC-SD cells with stable PLEK2 knockdown (a) and PLEK2 overexpression (c) were measured by transwell migration and matrigel invasion assays. b, d Represent images of mouse livers of NOZ cells with stable PLEK2 knockdown (b) and PLEK2 overexpression (d) are shown and the numbers of metastatic nodes per liver were measured. e Morphological change of NOZ cells upon PLEK2 knockdown or overexpression. f Expression of epithelial marker E-cadherin, mesenchymal markers Fibronectin, Vimentin and N-cadherin were detected by western blot. All experiments were repeated at least three times, and data were analyzed using student’s t-test. * P < 0.05. Error bars indicate SEM