Fig. 6.

CCL2 was a target gene downstream of PLEK2/EGFR signaling. a Differential gene pathway analyses of PLEK2 knockdown cells by mRNA sequencing. b, c The relative mRNA levels of cytoskeleton organization genes were detected by qRT-PCR. d Protein levels of CCL2 in indicated cell lines with either PLEK2 knockdown or overexpression were detected by western blot. e Protein levels of CCL2 in PLEK2 overexpression cells with EGFR knockdown were detected by western blot. f The protein levels of secreted CCL2 in cellular supernatant were detected by ELISA analysis. g Migration and invasion ability of PLEK2 knockdown NOZ cells with 0.1μg/mL CCL2 treatment were measured by transwell. h Protein levels of EMT markers in PLEK2 knockdown cells with 0.1μg/mL CCL2 treatment for 24 h were detected by western blot. i The relative mRNA levels of EMT related transcription factors in PLEK2 knockdown cells with 0.1μg/mL CCL2 treatment for 24 h were detected by qRT-PCR. All experiments were repeated at least three times, and data were analyzed using student’s t-test. * P < 0.05. Error bars indicate SEM