Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 10;19:555. doi: 10.1186/s12885-019-5769-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 5 — Confirmation the effect of SNX27 knockdown on E-cadherin activity by siRNA transfection. SNX27-KD and WT cells (50–60% confluence in 12-well plates) were transfected with E-cadherin siRNA. Lysates were blotted with anti-E-cadherin, anti-PCNA and anti-EMT-related markers. The SNX27-KD MDA-MB-231 cells transfected with E-cadherin siRNA were significantly decreased the E-cadherin expression compared with cells treated with control siRNA and transfection reagent control. The expression of PCNA, the cell proliferation marker, was increased in SNX27-KD cells transfected with E-cadherin siRNA. Data are analyzed with One-way ANOVA adjusting multiple comparisons with Turkey method, and expressed with mean ± SD from six independent experiments (n = 6). ns: no significantly difference, *p < 0.05, **p < 0.01, ***p < 0.001