Fig. 7.

Expression of critical EMT regulators in tumors from nude mice. a SNX27-KD MDA-MB-231 cells accelerated the protein expression of E-cadherin and β-catenin, and decelerated the protein expression of Vimentin and Claudin-5 in the tumors isolated from the nude mice model injected with breast cancer cells. Data are analyzed with Welch’s t-test, and expressed with mean ± SD from three independent experiments (n = 3). *p < 0.05 vs. WT control. **p < 0.01 vs. WT control. b Immunofluorescence staining of β-catenin and Vimentin was shown that these proteins were expressed in tumors from SNX27-KD injected mice and WT MDA-MB-231 breast cancer cells. Scale bar is 10 μm. Duplicate samples of each wafer were included in three independent experiments (n = 3). c Cell proliferation in the tumor tissue of mice injected with SNX27-KD cells were decelerated compared with wildtype cells by immunofluorescence staining of BrdU (scale bar is 20 μm). BrdU index (number of cell stained with BrdU / number of total cell) was significantly higher in mice injected with wildtype MD-MB-231 cells compared with SNX27-KD cells. Duplicate samples of each wafer were included in three independent experiments (n = 3). **p < 0.01 vs. WT control