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. 2019 Jun 4;10:289. doi: 10.3389/fpsyt.2019.00289

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Copyright © 2019 Yang, Sun, Wu, Lu, Wang, Li, Zhou, Sun and Sun

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) The process diagram of the experiment. SPS was conducted in three stages. Rats were immobilized for 2h (IMO 2 h), forced to swim for 20 min (FS 20 min), allowed to recover with a heating lamp for 15 min, and then exposed to diethyl ether until loss of consciousness (Ether). Rats were then returned to their home cages and left undisturbed for 7 days until the experimental manipulations. (B) The experiment included the control group (n = 14), PTSD group without MS (n = 14), PTSD-treated with prior 3h MS (n = 14), PTSD-treated with prior 6h MS (n = 14) or simply ‘Control,’ ‘PTSD,’ ‘MS3h-PTSD’ and ‘MS6h-PTSD’, respectively. Behavior experiments included OFT, EPMT, WMT and FST.