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. 2018 Nov 21;12(4):611–619. doi: 10.1111/1751-7915.13340

Figure 2.

Figure 2

Production and effect of AHL produced by K. rhaeticus Sender strains (A) Testing the ability of K. rhaeticus Sender to produce AHL. The left illustration demonstrates the process of testing the AHL concentration in K. rhaeticus spent media. The right chart shows the mean mRFP production rate response from an E. coli Receiver assay after the addition of fresh HS media and spent media from K. rhaeticus Empty and K. rhaeticus Sender. Error bars denote the standard deviation from three repeats.B. Dose–response of K. rhaeticus Receiver 2 h post‐treatment with increasing concentrations of AHL. The response is measured in fluorescence units and is normalized with a media blank on the same plate. n = 3 for all concentrations except for 0.1 nM AHL where n = 2.C. The mean mRFP fluorescence response of liquid K. rhaeticus Receiver 1‐h postaddition of spent media from K. rhaeticus Sender. The left illustration details the process of adding spent media to K. rhaeticus and the method used to measure the fluorescence response. Treated strains included K. rhaeticus Receiver, the original pLux01 vector and K. rhaeticus with pEmpty. Error bars denote the standard deviation from three repeats.