Table 3.
Distribution of resistance genes in E. coli strain EC25
| Resistance gene | %identity | HSP length/query | Contig | Position in contig | Predicted phenotype | Accession number |
|---|---|---|---|---|---|---|
| Aminoglycoside | ||||||
| aph(3ʹ’)-Ib | 100 | 804/804 | pEC25-1 | 94,244..95,047 | Aminoglycoside resistance | AF321551 |
| aph(6)-Id | 100 | 837/837 | pEC25-1 | 95,047..95,883 | Aminoglycoside resistance | M28829 |
| aac(3)-IId | 99.88 | 861/861 | pEC25-1 | 97,435..98,295 | Aminoglycoside resistance | EU022314 |
| aadA16 | 99.65 | 846/846 | pEC25-1 | 111,488..112,333 | Aminoglycoside resistance | EU675686 |
| aac(6ʹ)-Ib-cr | 100 | 600/600 | pEC25-1 | 113,669..114,268 | Fluoroquinolone and aminoglycoside resistance | DQ303918 |
| aadA5 | 100 | 789/789 | pEC25-1 | 204,055.204,843 | Aminoglycoside resistance | AF137361 |
| aac(6ʹ)-Ib-cr | 100 | 600/600 | pEC25-1 | 208,288.208,887 | Fluoroquinolone and aminoglycoside resistance | DQ303918 |
| Beta-lactam | ||||||
| blaNDM-7 | 100 | 813/813 | pEC25_NDM-7 | 44,052..44,864 | Beta-lactam resistance | JX262694 |
| blaOXA-1 | 100 | 831/831 | pEC25-1 | 209,018..209,848 | Beta-lactam resistance | HQ170510 |
| blaTEM-1B | 100 | 861/861 | pEC25-1 | 214,606.215,466 | Beta-lactam resistance | AY458016 |
| blaCTX-M-3 | 100 | 876/876 | pEC25-1 | 216,248..217,123 | Beta-lactam resistance | Y10278 |
| Fluoroquinolone | ||||||
| qnrB2 | 99.84 | 645/645 | pEC25-1 | 105,800..106,444 | Fluoroquinolone resistance | DQ351242 |
| aac(6ʹ)-Ib-cr | 100 | 600/600 | pEC25-1 | 113,669..114,268 | Fluoroquinolone and aminoglycoside resistance | DQ303918 |
| aac(6ʹ)-Ib-cr | 100 | 600/600 | pEC25-1 | 208,288..208,887 | Fluoroquinolone and aminoglycoside resistance | DQ303918 |
| qnrS1 | 100 | 657/657 | pEC25-1 | 221,088..221,744 | Fluoroquinolone resistance | AB187515 |
| Rifampicin | ||||||
| arr-3 | 100 | 453/453 | pEC25-1 | 113,120..113,572 | Rifampicin resistance | JF806499 |
| MLS - Macrolide, Lincosamide and Streptogramin B | ||||||
| mph(A) | 100 | 906/906 | pEC25-1 | 99,354..100,259 | Macrolide resistance | D16251 |
| mph(A) | 100 | 906/906 | pEC25-1 | 196,453..197,358 | Macrolide resistance | D16251 |
| Sulphonamide | ||||||
| sul2 | 100 | 816/816 | pEC25-1 | 93,368..94,183 | Sulphonamide resistance | HQ840942 |
| sul1 | 100 | 840/840 | pEC25-1 | 104,470..105,309 | Sulphonamide resistance | U12338 |
| sul1 | 100 | 840/840 | pEC25-1 | 110,191..111,030 | Sulphonamide resistance | U12338 |
| sul1 | 100 | 840/840 | pEC25-1 | 202,669..203,508 | Sulphonamide resistance | U12338 |
| Tetracycline | ||||||
| tet(A) | 100 | 1200/1200 | pEC25-1 | 118,110..119,309 | Tetracycline resistance | AJ517790 |
| Trimethoprim | ||||||
| dfrA27 | 100 | 474/474 | pEC25-1 | 112,514..112,987 | Trimethoprim resistance | FJ459817 |
| dfrA17 | 100 | 474/474 | pEC25-1 | 204,974..205,447 | Trimethoprim resistance | FJ460238 |