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. Author manuscript; available in PMC: 2019 Jun 11.
Published in final edited form as: Mol Cell Endocrinol. 2018 Jun 8;472:10–17. doi: 10.1016/j.mce.2018.06.003

Figure 4. Assessment of apoptosis in INS-1 cells.

Figure 4.

(A) Cell transfected with siRNA negative control or siRNA against FTO were stained with Annexin V-FITC and PI and analyzed by flow cytometry. (B) qPCR expression analysis of Ins1 and Ins2 genes in GRINCH cells transfected with negative control or siRNA against FTO. Cell lysate from transfected cells (with negative control or FTO) were used to measure GFP intensity as indicator for insulin content. Bars represent mean ±SD. * < 0.05.