Table II.
Primers used for mouse reverse transcription-quantitative polymerase chain reaction in the present study.
| Primer | Direction | Sequence, 5′ to 3′ | Tm, °C | Length, bp |
|---|---|---|---|---|
| SIRT1 | F | AGTTCCAGCCGTCTCTGTGT | 58.8 | 20 |
| R | GATCCTTTGGATTCCTGCAA | 52.4 | 20 | |
| SIRT2 | F | TAGACACGCTGGAACGAGTG | 57.2 | 20 |
| R | TCTCTTTCATCCAGCCCATC | 54.3 | 20 | |
| SIRT3 | F | CAGCTACATGCACGGTCTGT | 57.9 | 20 |
| R | ACACAATGTCGGGTTTCACA | 54.4 | 20 | |
| SIRT4 | F | CATCCAGCACATTGATTTCG | 51.7 | 20 |
| R | CCAGTCTCTCCCAGTTGCTC | 58.1 | 20 | |
| SIRT5 | F | CCATCACCCAGAACATTGACG | 53.7 | 20 |
| R | ACAGTGCCACACGAGGTACA | 59.0 | 20 | |
| SIRT6 | F | GTGGATGAGGTGATGTGCAG | 56.2 | 20 |
| R | TCAGCCTTGAGTGCTACTGG | 57.1 | 20 | |
| SIRT7 | F | GACTGAGCGTACTGCCCTTC | 58.5 | 20 |
| R | TTCAGAGGCTGCCCTAATGT | 56.1 | 20 | |
| β-actin | F | GGCTGTATTCCCCTCCATCG | 60.0 | 20 |
| R | CCAGTTGGTAACAATGCCATGT | 59.4 | 22 |
F, forward; R, reverse; SIRT, Sirtuin; Tm, melting temperature.