Table I.
The sequences of primers.
| Primer name | Direction | Sequence (5′-3′) |
|---|---|---|
| GAPDH | F | GAGTCAACGGATTTGGTCGT |
| GAPDH | R | CATGGGTGGAATCATATTGGA |
| E-cadherin | F | TACACTGCCCAGGAGCCAGA |
| E-cadherin | R | TGGCACCAGTGTCCGGATTA |
| Vimentin | F | AGGCAAAGCAGGAGTCCACTGA |
| Vimentin | R | ATCTGGCGTTCCAGGGACTCAT |
| MMP-9 | F | CTCTGGAGGTTCGACGTGAA |
| MMP-9 | R | TCAACTCACTCCGGGAACTC |
| TIMP-2 | F | CCAGAAGAAGAGCCTGAACCA |
| TIMP-2 | R | GTCCATCCAGAGGCACTCATC |
| miR-490-5p-RT | CTCAACTGGTGTCGTGGAGTC | |
| GGCAATTCAGTTGAGUCCACCCA | ||
| miR-490-5p | F | CATGGATCTCCAGGTGG |
| miR-490-5p | R | GAACATGTCTGCGTATCTC |
| TNKS2 | F | GTGAATGCCCAAGACAAAGGAGG |
| TNKS2 | R | GGTGTGAAAGCCCATTTGTCCG |
| MAP3K9 | F | AGGGTTCACCAGCCTTATGGAG |
| MAP3K9 | R | GGTGAATGCTGTAGGCGACTCT |
| NUFIP2 | F | AGCACCAGGAAACGCCGAAGAA |
| NUFIP2 | R | CTTGCTGGTTGCCATTGAGGAC |
| CLCC1 | F | TTCAGACTGGCAACAAGAGCCC |
| CLCC1 | R | TTCCACCTGTCTCCTTGGGCTT |
| ABCC2 | F | GCCAACTTGTGGCTGTGATAGG |
| ABCC2 | R | ATCCAGGACTGCTGTGGGACAT |
F, forward; R, reverse; RT, reverse transcription; miR, microRNA; MMP, matrix metalloproteinase; E, epithelial; TIMP-2, tissue inhibitor of metalloproteinase; MAPK, mitogen activated protein kinase.