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. 2019 Jun 4;10:1237. doi: 10.3389/fimmu.2019.01237

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Copyright © 2019 Soto-Dávila, Hossain, Chakraborty, Rise and Santander.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gene expression of (A) Interleukin 1b (IL-1b), (B) Interleukin 8 (IL-8), (C) Interleukin 10 (IL-10), (D) Major histocompatibility complex class 1 (MHC-I), (E) Goose type lysozyme (g-type lysozyme), (F) Bactericidal permeability increasing protein / lipopolysaccharide-binding protein (BPL/LBP), (G) Cathelicidin (CAMP), (H) Transferrin, (I) Hepcidin (HAMP), (J) Nuclear factor erythroid 2-related factor 2 (nrf2), (K) NADPH oxidase 1 (nox1), (L) Mn superoxide dismutase (Mn-Sod), (M) CuZn superoxide dismutase (Cu/Zn-Sod), and (N) Catalase (cat) in Atlantic cod primary macrophages isolated from head kidney and infected with live (J223) and formalin-killed A. salmonicida (FK 223) at different times post infection (1, 2, and 6 h). Relative expression was calculated using the 2(^−ΔΔCt) method and Log2 converted using EF-1a as internal reference gene. Different letters represent significant differences between primary macrophages infected with J223 strain (lower case) or inoculated with FK J223 (upper case) at different times-points. Asterisks (^*) represent significant differences between treatments on each time-point (^*p < 0.05, ^**p < 0.01, ^***p < 0.001). Each value is the mean ± S.E.M (n = 6).