Table 1.
Primer sequences used in this experiment.
| Gene | Forward (5′to 3′) | Reverse (5′to 3′) | Tm°C | Efficiency (%) | References |
|---|---|---|---|---|---|
| IL-1b | TGAGGACCTGCTCAACCTCT | TCTTCTGGTGGTCCCTCAAC | 55.6 | 103.7 | (35) |
| IL-8 | GGTTTGTTCAATGATGGGCTGTT | GACCTTGCCTCCTCATGGTAATACT | 56.5 | 98.4 | (36) |
| IL-10 | CCTATAAAGCCATCGGCGAGTTA | TGAAGTCGTCGTTTTGAACCAAG | 56.6 | 100.1 | (36) |
| MHC-I | CTAGCGTGGGACCTGAAGAC | CAGAGTGCTCTTCCCGTAGG | 56.5 | 108.4 | (35) |
| g-type lysozyme | CATTGACCAAGCCACTGGAATCCT | ATTCGACTCTACCGTCTCCAGTGT | 59.3 | 102.3 | (35) |
| BPI/LBP | GACCGTCAACGTGATGGCCCCGGT | CTTTGTTGGCCTCTATGCTGGAGAG | 59.4 | 96.8 | (37) |
| Cathelicidin (CAMP) | ATTGCAATTTCACCCTGAGC | CCAGACCTGCTCCTTCTCAC | 56.4 | 108.1 | (38) |
| Transferrin | GAGCTCCCATCGACAGCTAC | CAAACCCAGCAGAGGAGAAG | 56.7 | 108.9 | (39) |
| Hepcidin (HAMP) | CCACAGGCTCCTCTCAAGTC | CTGCAACTGCAATGCTGAAT | 56.4 | 105.1 | (38) |
| nrf2 | TCGCAGTAGGAGCTGGATGA | CTCCGGTCTGTCCTTGGAAA | 57.0 | 98.1 | (40) |
| nox1 | GCCTATATGATTGGCCTGATGAC | GCTGTGCTGAGTGGGTCGTA | 55.3 | 108.6 | (40) |
| Mn-Sod | ATGTGGCCTCCTCCATTGAA | GCATCACGCCACCTATGTCA | 55.1 | 109.2 | (40) |
| Cu/Zn-Sod | CATGGCTTCCACGTCCATG | CGTTTCCCAGGTCTCCAACAT | 56.8 | 98.0 | (40) |
| cat | GCCAAGTTGTTTGAGCACGTT | CTGGGATCACGCACCGTATC | 57.3 | 101.0 | (40) |
| EF-1a | GATGCACCACGAGTCTCTGA | GGGTGGTTCAGGATGATGAC | 56.2 | 98.3 | (35) |