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. 2019 May 8;44(1):196–206. doi: 10.3892/ijmm.2019.4187

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Copyright: © Zhao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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ZEB1-AS1 interacts with AUF1 to serve key roles in BCa. (A) Immunofluorescence analysis of AUF1 protein in UM-UC-3 cells. Scale bars, 10 µm. (B) The interaction between ZEB1-AS1 and AUF1 was confirmed by RNA pulldown assays and western blot analysis. GAPDH served as negative control. (C) RNA immunoprecipitation assays were performed using anti-AUF1 and control IgG antibodies, followed by RT-qPCR to examine the enrichment of ZEB1-AS1 and U6. U6 served as negative control. ^**P<0.01. (D) The silencing effect of si-AUF1 infection in BCa cells was determined by RT-qPCR (left panel) and western blot analysis (right panel). (E) Wound healing assays and (F) Transwell invasion assays were performed to detect the effect of AUF1 knockdown and ZEB1-AS1 overexpression on BCa cell migration and invasion, respectively. ^*P<0.05 vs. p-ZEB1-AS1+si-NC group. ZEB1-AS1, zinc finger E-box-binding homeobox 1-antisense 1; AUF1, heterogenous nuclear ribonucleoprotein D0; BCa, bladder cancer; RT-qPCR, reverse transcription quantitative polymerase chain reaction; si, small interfering; NC, negative control.