ZEB1-AS1 expression is associated with metastasis in patients with BCa. (A) ZEB1-AS1 expression level was detected by RT-qPCR in 60 BCa tissues and paired non-tumor tissues. (B) ZEB1-AS1 expression level was measured by RT-qPCR in 30 patients with MIBC and 30 patients with NMIBC. (C and D) ROC analysis was performed to investigate the diagnostic value of ZEB1-AS1 expression in differentiating between (C) patients with BCa and patients without cancer, and between (D) patients with MIBC from patients with NMIBC. (E) Correlation between RNA expression of ZEB1-AS1 and ZEB1 was analyzed from the Cancer Genome Atlas BLCA dataset using the online database StarBase. (F) The associations between the RNA expression of ZEB1-AS1 and ZEB1 in 60 BCa tissues were analyzed using Spearman's correlation analysis. ZEB1-AS1, zinc finger E-box-binding homeobox 1-anti-sense 1; BCa, bladder carcinoma; RT-qPCR, reverse transcription quantitative polymerase chain reaction; MIBC, muscle-invasive BCa; NMIBC, non-MIBC; AUC, area under the curve; BLCA, Bladder Urothelial Carcinoma.