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. 2019 May 21;44(1):145–156. doi: 10.3892/ijmm.2019.4204

Figure 3.

Figure 3

Oxidative stress in (A) HPNE and (B) AsPC-1 cells following exposure to CdCl2. Cells were grown and plated as described in the methods section. After loading with 100 μM DCFH, cells were washed twice with warm PBS and baseline fluorescence was measured (time 0). Treatment groups of (0 μM, PBS-only), and 50 or 100 μM CdCl2 were added to the appropriate treatment wells. DCF fluorescence was quantified at 30, 60, 90 and 120 min using a BioTek plate reader set at 485 nm(ex)/530 nm(em). The RFU values expressed were corrected for background (35-48 RFU) for each group. There was a significant effect of treatment-concentration in both HPNE (F2,105=10.45; P<0.0001) and AsPC-1 (F2,105=16.45; P<0.0001) cells. Data are expressed as the means ± SEM of 8 assays (n=8) performed in duplicate. CdCl2, cadmium chloride. *P<0.01 compared to the corresponding 0-time-point.