Figure 4.

Mitochondrial toxicity following exposure to various concentrations of CdCl₂ in the presence of 25 mM glucose or 10 mM galactose. Cells were maintained in regular growth media until assayed. Cells were exposed to 0.01-100 μM CdCl₂ for 4 h and the number of viable cells was estimated by MTT assay in the (A) HPNE and (B) AsPC-1 cells. Note the slight leftward shift in the curves for the 10 mM galactose group indicating a greater sensitivity to CdCl₂ toxicity. Data are expressed as the means ± SEM of 12 assays (n=12) performed in duplicate and represent a composite of all assays analyzed by nonlinear curve fitting - single site inhibition analysis. CdCl₂, cadmium chloride.