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. 2019 May 21;44(1):145–156. doi: 10.3892/ijmm.2019.4204

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Copyright: © Wallace et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Measurement of mitochondrial permeability using JC-1 dye following exposure to 1 μM CdCl₂ in HPNE and AsPC-1 cells. Fluorescence intensity was measured at 535ex/595em to measure JC-1 aggregates (healthy cells; green) and then a second reading using 485ex/535em which measures JC-1 monomers (apoptotic/unhealthy cells; red). The ratio of green/red represents a relative number of cells which are 'healthy'. There was a significant effect of treatment (F_1,16=7.349; P=0.0154) with reductions in the ratio following exposure to 1 μM CdCl₂ in both groups. Individual comparisons to control values did not reveal any difference in the fluorescent ratio in either cell line. Data represent the means ± SEM of 5 assays (n=5) performed in duplicate. CdCl₂, cadmium chloride.