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. 2019 May 13;44(1):227–239. doi: 10.3892/ijmm.2019.4195

Figure 2.

Figure 2

BLM induces endoplasmic reticulum stress in MLE-12 cells. (A) Immunofluorescence staining for pro-SP-C (red) in MLE-12 cells. DAPI (blue) staining was used to detect the nuclei (magnification, ×400). (B) Following exposure to the indicated concentrations of BLM for 24 h, the viability of MLE-12 cells was measured by Cell Counting kit-8 assay. (C) Effects of various concentrations of BLM stimulation for 24 h on LDH activity in the supernatants of MLE-12 cells. (D) Western blot analysis was used to detect the protein expression levels of XBP1 and Bip in MLE-12 cells treated with various concentrations of BLM for 24 h. (E and F) Semi-quantitative analysis of XBP1 and Bip protein expression levels. Data are presented as the means ± standard error of the mean, n=3. *P<0.05, **P<0.01, ***P<0.001 vs. 0 μg/ml BLM; #P<0.05 vs. 0.1 μg/ml BLM. Bip, immunoglobulin heavy chain-binding protein; BLM, bleomycin; LDH, lactate dehydrogenase; MLE, mouse lung epithelial; OD, optical density; XBP1, X-box binding protein 1.