Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 13;44(1):227–239. doi: 10.3892/ijmm.2019.4195

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Peng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

BLM inhibits proliferation and promotes apoptosis of MLE-12 cells. (A) Exposure to 1 μg/ml BLM for 24 h; Ki67 (red) expression was detected in MLE-12 cells by immunofluorescence staining. DAPI (blue) staining was used to detect the nuclei (magnification, ×400). (B) Semi-quantitative analysis of Ki67-positive MLE-12 cells. (C) Apoptotic cells were detected by Annexin V-fluorescein isothiocyanate/PI staining and flow cytometry. (D) Quantitative analysis of total apoptotic Annexin V⁺/PI⁻ and Annexin V⁺/PI⁺ cells is shown. Data are presented as the means ± standard error of the mean, n=3. ^**P<0.01, ^***P<0.001 vs. Con. BLM, bleomycin; Con, control; MLE, mouse lung epithelial; PI, propidium iodide.