Skip to main content
. Author manuscript; available in PMC: 2019 Jun 11.
Published in final edited form as: Cell Rep. 2019 Apr 4;27(3):940–954.e6. doi: 10.1016/j.celrep.2019.01.009

Figure 4. ST6Gal-I Is Present in Exomeres and in a Subset of Exosomes.

Figure 4.

(A) Immunoblot of ST6Gal-I and BACE1 in exosomes and exomeres with the indicated antibodies. Equal amounts of proteins from exosomes and exomeres were loaded. m, membrane; s, soluble.

(B) FAVS analysis of exosomes derived from DiFi cells. Dot plot of baseline fluorescent intensities from FAVS analysis of exosomes. No stain (upper left). Total fluorescent intensities from FAVS analysis of exosomes stained with a phycoerythrin-labeled CD81 antibody (x axis) and an Alexa 647-labeled EGFR antibody (cetuximab [CTX]) (y axis) (upper right). Percentages of gated populations from 10,000 total events are shown. Post-sort analysis of double-stained low-intensity (red box, lower left) and high-intensity (blue box, lower right) events.

(C) Immunoblot of flow-sorted exosomes. The same number of sorted vesicles were lysed, separated on a SDS/PAGE gel, and immunoblotted with the indicated antibodies. m, membrane; s, soluble.

See also Figure S5.