Table 2.
Compound | Calibration range | LLOD (ng/ mL) | LLOQ (ng/ mL) | Linear dynamic range | R2 | Spiked amount (ng/ mL) | Accuracy (%) | Precision (%) | Recovery (%) |
---|---|---|---|---|---|---|---|---|---|
GA | Low | 0.35 | 1.02 | 5.100–2040 | 0.998 | 200 | −3.82 | 4.38 | 115 (8.82) |
High | 2040–6120 | 0.995 | 2000 | −17.1 | 6.53 | 121 (10.2) | |||
3,4-diHBA | Low | 0.62 | 2.55 | 2.560–2040 | 0.998 | 200 | 3.31 | 3.21 | 92.0 (7.41) |
High | 2040–6120 | 0.999 | 2000 | 7.49 | 3.65 | 102 (5.32) | |||
3,4-diHPAA | Low | 1.23 | 5.01 | 2.652–2080 | 0.999 | 200 | −15.5 | 8.96 | 108 (12.0) |
High | 2080–6240 | 0.996 | 2000 | −17.7 | 4.13 | 116 (6.34) | |||
4-HBA | Low | 0.29 | 0.97 | 1.325–1060 | 0.999 | 200 | – | – | – |
High | 1060–6360 | 0.998 | 2000 | −9.23 | 4.55 | 102 (1.87) | |||
( + )-c | Low | 0.41 | 1.32 | 1.325–1060 | 0.999 | 200 | 4.57 | 9.69 | 91.2 (2.78) |
High | 1060–6360 | 0.996 | 2000 | 2.89 | 4.54 | 95.0 (6.44) | |||
3,4-diHPPA | Low | 0.51 | 2.60 | 5.204–1040 | 0.998 | 200 | −8.75 | 12.2 | 110 (9.43) |
High | 1040–6240 | 0.997 | 2000 | −20.8 | 3.16 | 117 (6.56) | |||
HA | Low | 0.42 | 1.29 | 2.505–1002 | 0.999 | 200 | – | – | – |
High | 1002–6012 | 0.998 | 2000 | −3.57 | 3.77 | 114 (9.02) | |||
VA | Low | 0.75 | 2.36 | 2.602–1040 | 0.999 | 200 | 5.82 | 0.98 | 104 (2.84) |
High | 1040–6240 | 0.999 | 2000 | 2.21 | 4.05 | 102 (8.04) | |||
CA | Low | 0.25 | 1.08 | 2.464–980.1 | 0.999 | 200 | −11.0 | 9.55 | 102 (2.90) |
High | 980.1–6020 | 0.997 | 2000 | −7.81 | 4.44 | 108 (4.15) | |||
( − )-EC | Low | 0.51 | 1.60 | 1.275–1020 | 0.999 | 200 | 12.6 | 5.81 | 93.6 (4.59) |
High | 1020–6120 | 0.998 | 2000 | 14.3 | 5.35 | 91.1 (7.18) | |||
HVA | Low | 0.63 | 1.62 | 2.700–2160 | 0.999 | 200 | −3.28 | 5.90 | 100 (5.83) |
High | 2160–6480 | 0.999 | 2000 | −6.48 | 1.58 | 106 (2.62) | |||
3-HBA | Low | 1.25 | 3.75 | 2.452–1020 | 0.999 | 200 | −0.67 | 5.12 | 108 (5.42) |
High | 1020–6120 | 0.998 | 2000 | −1.96 | 2.81 | 102 (2.25) | |||
3-HPAA | Low | 0.74 | 2.07 | 2.718–1040 | 0.999 | 200 | −3.10 | 4.33 | 106 (6.78) |
High | 1040–6240 | 0.999 | 2000 | −1.85 | 1.76 | 94.6 (4.10) | |||
diHCA | Low | 2.05 | 8.11 | 10.80–1080 | 0.997 | 200 | −15.9 | 4.70 | 112 (5.45) |
High | 1080–6480 | 0.995 | 2000 | −19.9 | 2.19 | 116 (4.73) | |||
p-CA | Low | 0.22 | 0.69 | 1.245–996 | 0.999 | 200 | −1.24 | 1.89 | 101 (5.14) |
High | 996–5976 | 0.996 | 2000 | −5.74 | 4.22 | 100 (3.45) | |||
FA | Low | 0.50 | 1.51 | 1.374–1080 | 0.999 | 200 | 4.96 | 4.67 | 96.6 (5.80) |
High | 1080–6480 | 0.999 | 2000 | 6.04 | 7.84 | 93.6 (5.15) | |||
3-HPPA | Low | 0.31 | 2.65 | 5.306–1060 | 0.999 | 200 | – | – | – |
High | 1060–5360 | 0.998 | 2000 | 4.27 | 3.19 | 90.2 (4.94) | |||
4-HPVA | Low | 0.76 | 2.61 | 2.607–1040 | 0.999 | 200 | 6.20 | 3.40 | 94.4 (4.13) |
High | 1040–5240 | 0.996 | 2000 | 8.25 | 2.93 | 96.8 (2.20) | |||
trans-Cinnamic acid-d7 (I.S.) | 0.23 | 0.71 | 1.272–1018 | 0.999 | – | – | – | – |
Data are expressed as means of six independent replicates. Standard deviations for Recoveries (%) were shown in parentheses.
Abbreviations are the same as in Table 1. LLOD, lower limit of detection; LLOQ, lower limit of quantification.
Calibration concentrations were divided into two ranges (low, ca. 1.25–1000 ng/mL and high, ca. 1000–6000 ng/mL), with optimum fit-to-line linear response. Some values for the 200 ng/mL spiked samples were null (−) due to the high response from the amount of the compounds existing endogenously.