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. Author manuscript; available in PMC: 2019 Jun 11.
Published in final edited form as: J Invest Dermatol. 2017 Sep 20;138(2):423–433. doi: 10.1016/j.jid.2017.08.043

Figure 5. Topically applied recombinant Hsp90α-Δ protein promotes wound closure as effectively as Hsp90α-wt protein.

Figure 5.

(a) Recombinant mouse Hsp90α-Δ and mouse Hsp90α-wt proteins were produced from cDNAs isolated from the dermal fibroblasts of the mice by pET bacterium inducible expression system, purified by fast protein liquid chromatography and visualized on a Coomassie blue-stained SDS gel. (b) Colloidal gold migration assay of human keratinocytes under the indicated stimulations. (c) Images of wound closure in Hsp90α-wt mice under carboxymethyl cellulose, Hsp90α-Δ, or Hsp90α-wt protein treatment. The yellow arrow indicates the relative distance of the yet unhealed portion of a wound. (d) Quantitation of the triplicate wounds from the experiment shown in (c), n = 3. *P ≤ 0.05. Scale bar = 1.0 cm. FBS, fetal bovine serum; Hsp, heat shock protein; wt, wild type.