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(A) by incubation of 7 μg of the enzymes with 80 mM pyruvate and 20 mM ManNAc in 125 mM of HEPES pH 8.0 and (B) by incubation of 7 μg of the enzymes with 80 mM pyruvate and 20 mM GlcNAc in 125 mM of CAPS pH 11.0. AsNAL is shown with withe bars and EcNAL with black bars.
